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Spectral Methods


Fluorescent Analysis

Objects of study

  • Liquids and solids, organic and inorganic substances.
  • Reactions.

Application

  • Substance determination: high technique sensitivity allows to determine the small substance concentrations and estimate the degree of substance purity.
  • Study of the rapid reactions of excited molecules, acid-alkaline reactions of the excited molecules and excited electron state complexing. Due to a high sensitivity the method permits to determine a low degree of conversion and elucidate the mechanism of chemical reaction by the intermediates.
  • In biology – study of the protein structure by the method of fluorescent probes and tags.

Instrument facilities

RF-5000 Spectrofluoremeter (Shimadzu, Japan). The wavelength range of the excitation monochromator is 200–700 nm. The wavelength range of fluorescence monochromator is 200-700 nm. The xenon lamp with a capacity of 150 wt serves as an emitter. Recording of the spectra of emission, excitation, synchronous spectra is accomplished.

Leading scientists

Prof. D.I. Kochubey.
Dr. G.V. Odegova.



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